David CALLIGARIS

En résumé

Entreprises

• Bayesian Ventures/inviCRO, LLC - Mass Spectrometry Consultant

  2013 - maintenant - Analyze samples by mass spectrometry imaging and report the data to drug discovery teams of pharmaceutical and biotechnology companies
  - Extensively evaluated the analytical performance of mass spectrometers as a device for intraoperative diagnosis at multiple vendor’s sites
  

• Brigham and Women's Hospital/Harvard Medical School - Postdoctoral Research Fellow

  2012 - maintenant - Collaborate and coordinate with faculty and students across departments to conduct interdisciplinary research
  - Perform drug development research by proteomics, lipidomics, MALDI mass spectrometry imaging, liquid surface extraction analysis, and fragment based drug discovery
  - Validate DESI mass spectrometry as an intraoperative tool for real-time diagnosis during tumor surgery in the Advanced Multimodality Image Guided Operating suite at Brigham and Women’s Hospital
  - Develop a platform aiming to study by MALDI imaging mass spectrometry coupled to fluorescence microscopy tumor cells isolated on an on-chip culture system
  

• Groupe Interdisciplinaire Mass Spectrometry Laboratory/Liège Universityde Génoprotéomique Appliquée - Postdoctoral Research Fellow

  2011 - 2012 - Introduced FTICR mass spectrometry for protein identification by MALDI in-source decay imaging
  - Studied breast tumor heterogeneity by MALDI mass spectrometry imaging
  - Characterized low-abundant phospholipid species markers of necrosis, inflammation and proliferating cells in breast cancer samples by LC-ESI-MS/MS
  - Directed one undergraduate and one graduate students’ researches and supervised their thesis
  

• Aix-Marseille University - Doctoral Fellow

  2007 - 2011 - Characterized tubulin isotypes by MALDI in-source decay mass spectrometry in solution and in situ
  - Prepared custom peptides using solid phase synthesis in a GMP/GLP environment
  - Developed an approach to study by affinity proteomics microtubule plus-end tracking proteins interacting with end-binding 1 protein C-terminus
  - Lectured, evaluated work, and advised undergraduate and graduate students during their internship
  

• Paul Sabatier University Toulouse 3 - Graduate Research Assistant

  2006 - 2007 - Performed cell culture and differentiation of promyelocytic NB4 cells
  - Analyzed apoptotic response of bortezomib-treated NB4 cells by flow cytometry
  - Performed tests of NB4 cell growth in SILAC medium containing and proteomic analyses by LC-ESI-MS/MS of heavy/light amino acid incorporation into NB4 cell proteome
  

• Victor Segalen University Bordeaux 2 - Graduate Research Assistant

  2005 - 2005 - Selected scFv candidates against microglial cells targets by phage display for further reformatting into IgG1

• Bristol-Myers Squibb - Undergraduate Research Assistant

  Rueil-Malmaison cedex 2004 - 2004 - Analyzed drugs by UV-visible/IR, atomic spectroscopy and GC in a GLP environment
  - Was in charge of the pharmaceutical library (drug listing, ordering, storage)
  

Formations

Pas de formation renseignée

Réseau

• Anaïs CALLIGARIS

• Fabrice CATTANT

• Florence TROUILLOT

• Guiet ROMAIN

• Jan GÜNTHER

• Jennifer BRU

• Kareen RICHARD

• Sandrine UTTENWEILER

• Stéphane MOREAU

• Stéphanie HEIL (DA SILVA)